Liquid nitrogen tank operation training: an advanced guide from novice to expert
Editor:adminClick：
Time:2025-03-25 13:46
Liquid nitrogen tanks are commonly used low-temperature storage equipment in laboratories, medical and industrial fields. Correct operation can not only ensure the safety of the solution, but also extend the service life of the equipment. This guide will take you from basic operation to advanced skills, and gradually master the use of liquid nitrogen tanks.
1. Beginner's guide: basic operation
First use inspection
Check whether the appearance of the liquid nitrogen tank is dented, damaged or rusted.
Confirm that the vacuum seal is good (you can judge whether the tank body is frosted. If the outer wall is frosted, the vacuum may work).
Make sure that the supporting tools (buckets, liquid nitrogen pumps, etc.) are complete.
Liquid nitrogen film
Wear antifreeze gloves and goggles to avoid frostbite caused by liquid nitrogen splashing.
Inject liquid nitrogen slowly to avoid blinking and splashing.
It is recommended to let it stand for 1-2 hours before formal use for the first filling, and the shampoo tank adapts to the low temperature.
Sampling
Use a special basket or take and put liquid from the sub to avoid manual operation.
Immediate action should be taken to reduce the time of liquid nitrogen absorption and injection.
2. Advanced skills: efficient management and maintenance
Liquid nitrogen level monitoring
Regularly check the remaining liquid nitrogen level (at least once a week) to avoid liquid damage caused by aging of liquid nitrogen.
Real-time monitoring can be achieved using a liquid nitrogen ruler or electronic liquid level alarm.
Reduce liquid grid gain
Try to reduce the number of times the lid is opened and the time of one-time opening.
Choose a liquid nitrogen tank with a locking lid or good vacuum sealing to reduce the evaporation rate.
Long-term storage optimization
Samples should be stored in categories and clearly marked to avoid repeated searches.
In addition, for long-term storage, it is recommended to choose a liquid nitrogen tank with high vacuum insulation performance to reduce the frequency of refilling.
3. Expert-level operation: troubleshooting and safety accident response
Common problem handling
Frosting of the storage tank: It may be that the vacuum layer fails, and the manufacturer needs to be contacted for repair.
Valve leakage: Check whether the sealing ring is aging and replace it in time.
Handling accidents
Liquid nitrogen leakage: Ventilate immediately to avoid inhaling high concentrations of nitrogen that may be caused.
Frostbite treatment: quickly soak the damaged part with warm water (about 40℃), and seek medical attention if it is serious.
Regular maintenance
Check the vacuum and sealing every six months.
Perform professional warranty once a year to ensure that the equipment is in the best condition.
4. Summary
Mastering the correct operation of liquid nitrogen tanks can not only improve experimental efficiency, but also ensure the safety of personnel and samples. From novice to expert, the key lies in standardized operation, regular maintenance, and preparation. If you have more complex usage requirements, please feel free to consult professional technicians!
#Liquid nitrogen tank #Laboratory safety #Cryogenic storage #Operation guide
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