Liquid nitrogen tank cell storage
Editor:adminClick:
Time:2018-07-25 09:28
Our company mainly produces liquid nitrogen container for high-tech products.Liquid nitrogen tank can be devided into storage liquid nitrogen biological container and transport liquid nitrogen biological container,which are used for frozen storage of fine livestock semen, cryopreservation of human organs and cells and so on.
With the development of science and technology and the needs of industrial and agricultural production, it will be rapidly promoted and applied, and will show a bright future. Today we mainly introduce “Liquid nitrogen tank cell storage.”
Liquid nitrogen tank cell storage
How a large number of cells are stored in a liquid nitrogen tank, the liquid nitrogen tank is generally filled with cryogenic liquid nitrogen, the storage of the cells requires a low temperature environment, and the liquid nitrogen in -196 degrees is a suitable environment for long-term preservation of the cells. The conventional liquid nitrogen tank stores the cells in the liquid phase of the liquid nitrogen tank. There is a special square drawer type basket. The 2ml cryotube is placed in the cryostat and then placed in the basket and immersed in liquid nitrogen to be completely immersed. The cells can be preserved for a long time by keeping the cells completely in a liquid nitrogen-196 degree environment.
However, the storage of liquid nitrogen tanks is divided into two types, and one is gas phase storage. If the gas phase is stored, the temperature is higher than the liquid phase storage, but it can reach -190 degrees, which is enough for cell storage. . Moreover, it can solve some problems that have long appeared and are difficult to avoid in liquid storage cells, such as sample contamination, canister bursting, and the like. Relatively speaking, the liquid nitrogen tank has the same volume of liquid nitrogen tanks for a longer period of time than the gas phase. The temperature of the vapor phase is slightly higher, but the same volume of liquid nitrogen tanks are stored for a shorter period of time ( The amount of liquid nitrogen is small, so the frequency of adding liquid nitrogen is higher.) The gas phase requires frequent addition of liquid nitrogen.
Liquid nitrogen tank cell storage
The problem of liquid-phase preservation of cells is difficult to solve. If the cryotube is not well sealed in the tank, liquid nitrogen is infiltrated into the tube, and the entire sample tank is filled. When the sample tank is taken out, the liquid nitrogen boils two-phase change pressure. Elevation, blasting, especially glass ampoules, is more dangerous. Therefore, it is now necessary to use a plastic sample tube with a sealing ring and a sample tube for the ultra-low temperature of the liquid nitrogen tank. Prevent tube and tank contamination. Therefore, if conditions are met, it is recommended to use a gas-liquid nitrogen tank. The gas phase is generally safer. The problem of refilling often needs to be solved by a compatible liquid-filled tank. There is also an automatic rehydration module that can be installed with automatic rehydration, without the need for human guards. It is safe for both the sample and the operator.
Liquid nitrogen tank cell storage
If you are interested in the liquid nitrogen containers or need to consult, please click on our online customer service, or call us. Pan Chao, Supply and customize different liquid nitrogen tanks, Pan Chao medical farming instruments, tailored for your different demands.
Welcome to send your inquiry, Phone number: +86 15038267159 Email: info@n2tank.com / sales@n2tank.com
Liquid nitrogen tank cell storage
With the development of science and technology and the needs of industrial and agricultural production, it will be rapidly promoted and applied, and will show a bright future. Today we mainly introduce “Liquid nitrogen tank cell storage.”
Liquid nitrogen tank cell storage
How a large number of cells are stored in a liquid nitrogen tank, the liquid nitrogen tank is generally filled with cryogenic liquid nitrogen, the storage of the cells requires a low temperature environment, and the liquid nitrogen in -196 degrees is a suitable environment for long-term preservation of the cells. The conventional liquid nitrogen tank stores the cells in the liquid phase of the liquid nitrogen tank. There is a special square drawer type basket. The 2ml cryotube is placed in the cryostat and then placed in the basket and immersed in liquid nitrogen to be completely immersed. The cells can be preserved for a long time by keeping the cells completely in a liquid nitrogen-196 degree environment.
However, the storage of liquid nitrogen tanks is divided into two types, and one is gas phase storage. If the gas phase is stored, the temperature is higher than the liquid phase storage, but it can reach -190 degrees, which is enough for cell storage. . Moreover, it can solve some problems that have long appeared and are difficult to avoid in liquid storage cells, such as sample contamination, canister bursting, and the like. Relatively speaking, the liquid nitrogen tank has the same volume of liquid nitrogen tanks for a longer period of time than the gas phase. The temperature of the vapor phase is slightly higher, but the same volume of liquid nitrogen tanks are stored for a shorter period of time ( The amount of liquid nitrogen is small, so the frequency of adding liquid nitrogen is higher.) The gas phase requires frequent addition of liquid nitrogen.
Liquid nitrogen tank cell storage
The problem of liquid-phase preservation of cells is difficult to solve. If the cryotube is not well sealed in the tank, liquid nitrogen is infiltrated into the tube, and the entire sample tank is filled. When the sample tank is taken out, the liquid nitrogen boils two-phase change pressure. Elevation, blasting, especially glass ampoules, is more dangerous. Therefore, it is now necessary to use a plastic sample tube with a sealing ring and a sample tube for the ultra-low temperature of the liquid nitrogen tank. Prevent tube and tank contamination. Therefore, if conditions are met, it is recommended to use a gas-liquid nitrogen tank. The gas phase is generally safer. The problem of refilling often needs to be solved by a compatible liquid-filled tank. There is also an automatic rehydration module that can be installed with automatic rehydration, without the need for human guards. It is safe for both the sample and the operator.
Liquid nitrogen tank cell storage
If you are interested in the liquid nitrogen containers or need to consult, please click on our online customer service, or call us. Pan Chao, Supply and customize different liquid nitrogen tanks, Pan Chao medical farming instruments, tailored for your different demands.
Welcome to send your inquiry, Phone number: +86 15038267159 Email: info@n2tank.com / sales@n2tank.com
Liquid nitrogen tank cell storage
Liquid nitrogen tank | cryogenic liquid nitrogen | Liquid nitrogen container |
Liquid nitrogen tank | cryogenic liquid nitrogen | Liquid nitrogen container |
Liquid nitrogen tank | cryogenic liquid nitrogen | Liquid nitrogen container |
Liquid nitrogen tank | cryogenic liquid nitrogen | Liquid nitrogen container |
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