Liquid nitrogen tank is widely used in six livestock frozen with improved skills in a variety of six animals, cattle semen frozen preparation, survival skills, since the last century 50 years has formed a complete set of process.
  Bovine semen Frozen cold source widely used liquid nitrogen. Granules of semen in the liquid nitrogen cooled by the fluorine plate (PTFE), copper gauze, aluminum on the frozen. To allow the surface of the semen to be connected with the liquid nitrogen surface - the interval (1 ~ 2 cm). In the course of freezing, to maintain the temperature at -80 ~ -120 ℃. Before the freeze will be balanced after the semen enrichment, and check the sperm viability. Drops to be agile, the particles should be symmetrical, diluted by 10 cents per milliliter of fine drops is appropriate After the end of the freeze, to pause for 2 to 3 minutes until all the particles have been frozen immediately put into liquid nitrogen. Sampling check (the same is usually randomly selected 2), thaw rate of 0.3 or more, you can install in the gauze bag, after the mark in the liquid nitrogen to survive. Each drop of ice after a bull's semen, you must swap the fluoride plate and other appliances. Now, the capacity of the tube is 0.25 ml and 0.5 ml, made of non-toxic plastic (polyvinyl chloride). The end of the tube filled with tampons and polyethylene powder, the powder is cured by water that active sealing, when the infusion of refined semen to become the piston; the other end of the injection of semen, you can polyethylene or steel ball (or plastic beads) To be careful in the seal and the semen between 10 to 13 mm away from the space, to prevent the freezing process caused by expansion caused by thin tube burst.
  Sperm storage of frozen semen of liquid so liquid nitrogen to do cold storage, if necessary, can be removed at any time. In order to prepare for the impact of temperature changes on the quality of semen, take action to be agile, even if the elimination of the time in the atmosphere pause. When the frozen semen is extracted from the storage container, the semen should not override the neck of the liquid nitrogen container and stop the landing of the semen thawing rate due to the temperature rise. Cow frozen semen has been more than 40 years of history. Test proved that the survival of the frozen semen still has the ability to insemination. But the same is usually thought that the frozen semen of cattle with the extension of survival time, sperm vigor and insemination skills gradually low. Cow frozen semen long-term survival is simply cut the time limit, still need to inherit research and inspection.
  2, six animals and a variety of animals in the embryo transfer, the preparation of survival embryos
  Now more to accept the embryo freezing device, is an intelligent freezer. The instrument to accept the computer control skills, special software, can more accurately control the amount of liquid nitrogen emissions, so as to ensure that the frozen biological products to the appropriate freezing rate cooling frozen.
  3, liquid nitrogen ultra-low temperature collection of microbial skills
  The collection of bacteria at -196 ° C liquid nitrogen long-term collection method, it is the principle of the use of microorganisms in the following -130 ℃ metabolism tends to stop and useful collection of microorganisms. Large fungi are an important group in the fungi (a kind of fungus that forms a large fruiting body in the fungus, which refers to the mushrooms or mushrooms in the broad sense). Many species have high nutritional cost and medicinal cost. Some of the large-scale fungi can probably analyze the dead plants, to maintain the natural material circulation, ecological balance has an important role, can be opened up for the paper industry and environmental purification; some large-scale Fungi can cause tree disease or against a variety of wood products, the understanding of such pathogenic fungi to strengthen, is conducive to prepare and eliminate the occurrence of harm. Examples of large-scale fungi collection to deal with microbial strains of resources, quiet and efficient collection, and useful for long-term collection of genetic resources, remote collection of biodiversity sharing has great significance
  4, the survival of agricultural biological genes
  Shanghai invested more than 4100 million yuan to set up equipment to deploy China's first integrated agricultural bio-gene database. Agriculture industry, said the most promising market development potential of the seed industry, will be the gene pool as a source of breeding material. The total construction area of ​​3300 square meters of Shanghai agricultural biology gene pool, located in the Shanghai Academy of Agricultural Sciences, will be planted seeds, plant in vitro material, animal germ cells, microbial strains and plant genetic engineering materials five categories of agricultural biological genetic resources.
  5, the survival of liquid nitrogen vaccine
  Liquid nitrogen seedling is the middle and mid-80 years to gradually promote the use of the first use of type Ⅲ-FCl26 chicken chicken herpes virus liquid nitrogen seedling, and then the sea surface and developed SBl, 301 / B, Z4,814 and other liquid nitrogen vaccine, And combined into II + III type divalent seedlings and I + II + III trivalent seedlings, after the application were once useful to control some areas of chicken Marek's disease, II + III type divalent seed did not last long, 1993 Marek's disease in North America and the severe areas of the emergence of a wide range of prevalence, for the control of Marek's disease, some countries began to learn the Netherlands since 1927 using I-CVl988 / Rispens liquid nitrogen seedlings, the useful control of Marek's disease Curriculum vitae, the United States, Canada, Brazil, Japan, Europe have successively introduced from the Netherlands Ministry of Agriculture and Fishery CVl988 / Rispens seedlings, in their respective countries using the different skills of production, living in liquid nitrogen tank.
  6, with liquid nitrogen treatment of dairy cow milk nipple outside the mouth closed, six animal skin tumors are also more ordinary.
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