2 filling liquid nitrogen in time

 

The cryopreservation technology of livestock semen is to freeze semen by using the cooling capacity of liquid nitrogen, so that the sperm is in complete dormancy state, so as to achieve long-term preservation purposes. Therefore, the frozen semen should be immersed in the surface of the liquid, otherwise it is very easy to lose its vitality and failure. It is generally stipulated that liquid nitrogen should be supplemented when the amount of liquid nitrogen is reduced to 1 / 3 of the total capacity. There are 3 ways to check the amount of liquid nitrogen. (1) weighing method: weigh the liquid nitrogen tank in time and make a judgment. For example, 101, the net weight of the liquid nitrogen tank is about 8 k g, and the capacity is 8 k g. According to the regulation, liquid nitrogen should be supplemented when the liquid nitrogen is reduced to 1 /3 of the total capacity, so liquid nitrogen should be filled when the liquid nitrogen tank and the liquid nitrogen weight are less than 8.5 k g. At present, the liquid nitrogen tank inside the small bucket has been eliminated by the most improved, frozen semen into the bag with a rope hanging on the bottom of the liquid nitrogen tank, so the liquid nitrogen and the tank weight is 7.5 K above g reperfusion in liquid nitrogen can also be. If the freeze-cream is overloaded in the tank, it will bring trouble to the actual freezing fine tube. First, it is easy to make the bolt rope entangled together, and it is not easy to remove the thin tube. The two is that the frozen fine pipe has a certain weight and volume, which is not good for weighing the remaining liquid nitrogen in the tank. (2) the stick method: the bottom tank is inserted into the solid plastic sticks or solid sticks, 5 to 108 after the removal, the length is the height of frost. Do not use hollow rods or plastic tubes to prevent liquid nitrogen from injecting people into the tube. Long and thick wire can also be inserted into the tank slowly, and the general position of the liquid surface can be judged when the sound of the bubble is heard. (3) when the tank around the neck with sweating phenomenon or glaucous attachment, it is proved that the vacuum tank is not deteriorated, then, should be replaced immediately.

 

 

3 do a good leaning